Our laboratory strives to distribute published reagents and protocols in a timely manner by depositing them in public repositories or including them as supplemental data in our publications. These repositories provide easy access, a uniform MTA, and help coordinate any shipping and customs concerns that may arise. Additional information including legacy protocols, data files, and instructions are also available here.

This page lists only manuscripts with distributed reagents or protocols. Please also see our Hsiao Lab Publications page for a full listing of our work.

Resources linked to our publications were previously listed on Lablife; however, these pages are the most current versions.

Manuscripts

• 2008 Hsiao - NKX2-5 reporter - PLoSOne: Plasmids, Mice, and Cells: "Marking Embryonic Stem Cells with a 2A Self-Cleaving Peptide: A NKX2-5 Emerald GFP BAC Reporter" by Edward C. Hsiao, Yuko Yoshinaga, Trieu D. Nguyen, Stacy L. Musone, Judy E. Kim, Paul Swinton, Isidro Espineda, Carlota Manalac, Pieter J. deJong, Bruce R. Conklin. (PLoSOne 2008).

• 2008 Hsiao - Rs1 in bone - PNAS - Plasmids, Mice, and Protocols: "Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass" by Edward C. Hsiao, Benjamin M. Boudignon, Wei C. Chang, Margaret Bencsik, Jeffrey Peng, Trieu D. Nguyen, Carlota Manalac, Bernard P. Halloran, Bruce R. Conklin, and Robert A. Nissenson (PNAS 2008).

• 2011 Hsiao - Single Vector Tet - SCRT: Plasmids, Mice, Cells, and Protocols: "Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice" by Edward C. Hsiao, Trieu D. Nguyen, Jennifer K. Ng, Mark J. Scott, Wei Chun Chang, Hengameh Zahed, and Bruce R. Conklin. (SCRT 2011)
   
• 2012 Schepers, Hsiao - Gs signaling in osteoblastic cells and effects on HSCs: Protocols: "Activated Gs signaling in osteoblastic cells alters the hematopoietic stem cell niche in mice" by Koen Schepers*, Edward C. Hsiao*, Trit Garg, Mark Scott, and Emmanuelle Passegue (Blood 2012)

• 2013 Matsumoto, Schlieve, Hayashi - FOP iPS cells, Protocols: "Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation"  by Matsumoto Y, Hayashi Y, Schlieve CR, Ikeya M, Kim H, Nguyen TD, Sami S, Baba S, Barruet E, Nasu A, Asaka I, Otsuka T, Yamanaka S, Conklin BR, Toguchida J, Hsiao EC (OJRD 2013).

• 2014 Barruet, Hsiao: Protocols: Using Human Induced Pluripotent Stem Cells to Model Skeletal Diseases by Emilie Barruet and Edward C. Hsiao. (Methods Mol Biol. 2014). This manuscript details all of our iPS cell culture protocols.

Published Protocols (see indicated paper links above)

• IHC Protocols
  • Anti-FLAG: 2008 Hsiao - Rs1 in bone - PNAS
  • Anti-Osteocalcin (mouse): 2008 Hsiao - Rs1 in bone - PNAS
  • Anti-Osterix (mouse): 2008 Hsiao - Rs1 in bone - PNAS

• Mouse Genotyping Protocols
  • NKX2-5 BAC reporter: 2008 Hsiao - NKX2.5 reporter - PLoSOne
  • TetO-Rs1: 2008 Hsiao - Rs1 in bone - PNAS
  • ColI(2.3)-tTA: 2008 Hsiao - Rs1 in bone - PNAS

• FACS Protocols
  • Mouse MSCs, OBCs, and ECs: 2012 Blood

• IPS Cell Protocols
  • Feeder cell preparation: OJRD 2013; Methods Mol Bio 2014
  • FOP and control iPS cell maintenance culture: OJRD 2013; Methods Mol Bio 2014
  • Mineralization media culture and analysis: OJRD 2013; Methods Mol Bio 2014